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Foregut endoderm differentiation from Human H1 ESCs examining Retinoic Acid dependent patterning during day5-6 developmental window

GSE310903 Homo sapiens (human) RNA-seq (gene expression) 18 samples Submitted 2025/11/30 Platform GPL34284
At a glance

RNA-seq (gene expression) · human · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
18 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
To define Retinoic Acid regulated genes in Human ESC differentiated foregut endoderm, endoderm was treated with or without 2uM Retinoic Acid for 24 hours (day5-6) of differentiation protocol, and bulk RNA-seq was performed on day6 or day9. Treatment with 2uM CHIR99021 and 50ng/mL BMP4 for 72 hours (days6-9) done to mimic respiratory fate inducing signals.
This dataset
Open GSE310903 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE310903_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1367507 and SRA study SRP647486. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
6 conditions, mostly in triplicate
Human PSC Day6 foregut endoderm ×3 Human PSC Day9 foregut endoderm ×3 Human PSC Day9 foregut endoderm plus CHIR BMP ×3 Human PSC Day6 foregut endoderm plus RA ×3 Human PSC Day9 foregut endoderm plus RA ×3 Human PSC Day9 foregut endoderm plus RA plus … ×3

Supports a between-group comparison across 18 samples.

How we read this: 6 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.