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Distinct Control of Histone H1 Expression within the Histone Locus Body by CRAMP1

GSE310913 Homo sapiens Expression profiling by high throughput sequencing 3 samples Submitted 2026/01/30 Platform GPL34284
Summary
Proper histone gene expression is critical for cell viability and maintenance of genomic integrity. Multiple histone genes are organized into three genomic loci encode replication-coupled core and linker histones. Histone gene expression and transcript processing are orchestrated in the histone locus body (HLB) within the nucleus. We identified human CRAMP1 as a selective regulator of the linker histone H1 expression. CRAMP1 was recruited to the HLB in RPE1hTERT cells. Affinity purification showed that CRAMP1 physically associates with the HLB component GON4L (also known as YARP). We demonstrated that the PAH domains of GON4L interact with CRAMP1. CRAMP1 disruption resulted in a loss of histone H1 expression and a reduction in H1 protein levels. CRAMP1 occupies unmethylated promoters of replication-coupled linker histone genes that reside within the histone locus body and replication-independent histone H1 loci, which reside in a region of the genome without other histone genes. Together, these data identify CRAMP1 as a novel and selective regulator of histone H1 gene expression.
Published in
Human CRAMP1 specifically promotes the expression of histone H1 genes
Bodner J, Vadlamani P, Lee AS et al. · EMBO reports 2026 · PMID 41663757 · doi:10.1038/s44319-026-00704-9
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Also filed as BioProject PRJNA1367523 and SRA study SRP647507. Searching any of these in the dataset finder brings you back here.

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