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Enhanced immune response of human iPSC-macrophages via animal-free manufacturing processes

GSE311159 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/04/22 Platform GPL34284
Summary
Studying human macrophages in vitro is crucial for understanding their immune functions. Recent interest in macrophages derived from induced pluripotent stem cells (iPSC-Mac) has led to various differentiation protocols, often using undefined animal serum like fetal bovine serum (FBS). This study compared FBS-based media to the defined medium X-VIVO™ 15 containing human serum albumin (hSA). Both produced functional macrophages, but those in hSA displayed a neutral activation state, enhanced cholesterol metabolism, and more significant changes in surface phenotype after stimulation. They also had a stronger response to co-stimulatory signals, making them better for testing immunomodulatory drugs. In contrast, FBS-grown macrophages were more adherent and pre-activated, showing reduced sensitivity upon stimulation, which may pose challenges for drug screening and immunomodulatory evaluations.
Published in
Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screening
Schinke M, Gensch I, Rietschel T et al. · Stem cell research & therapy 2026 · PMID 41964059 · doi:10.1186/s13287-026-05009-1
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Direct links to NCBI, no account and no request form: the whole study as GSE311159_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1368751 and SRA study SRP648462. Searching any of these in the dataset finder brings you back here.

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