GEO series

A Novel Function of the Branched-Chain Fatty Acid iso15:0 in Suppressing HCC Malignant Progression through GPCR/PI3K/AKT Pathway Inhibition

GSE312291 Homo sapiens (human) RNA-seq (gene expression) 10 samples Submitted 2025/12/07 Platform GPL24676

At a glance

RNA-seq (gene expression) · human · 10 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Topic: Hepatocellular carcinomaThis series is part of our curated briefing on it.
Samples: 10 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Despite advances in hepatocellular carcinoma (HCC) treatment, the discovery of novel bioactive molecules with well-defined mechanisms remains a critical need. This study identifies the branched-chain fatty acid iso15:0 as a potent suppressor of HCC progression and delineates its underlying molecular pathway. We demonstrated that iso15:0 significantly inhibits the proliferation and migration of HCC cells in vitro and suppresses tumor growth and metastasis in vivo.

Read the full summary ↓

Transcriptomic profiling of treated cells revealed widespread alterations in gene expression, with Gene Set Enrichment Analysis (GSEA) pinpointing a significant suppression of the GPCR signaling pathway. Subsequent mechanistic investigations established that iso15:0 executes its function by specifically targeting the Gβγ subunits of heterotrimeric G proteins, thereby disrupting the downstream activation of the PI3K/AKT signaling cascade. This inhibition leads to cell cycle arrest and suppression of epithelial-mesenchymal transition (EMT). Our findings unveil a previously unrecognized tumor-suppressive function of iso15:0 and define a novel mechanism by which it inhibits HCC progression through targeted interference with the Gβγ–PI3K/AKT signaling axis. This work not only provides a mechanistic basis for the anti-tumor activity of iso15:0 but also highlights its potential as a promising lead compound for the development of new molecularly-targeted therapies against HCC.

This dataset

Open GSE312291 in finder → Find more human RNA-seq datasets → Hepatocellular carcinoma landscape →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE312291_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1372783 and SRA study SRP650847. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 10 more — browse all 10 samples with per-sample file links →

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GSE10143 Gene Expression in Fixed Tissues and Outcome in Hepatocellular Carcinoma 387 samples same disease briefing
GSE107170 Molecular Signature of Hepatitis D Virus-Associated Hepatocellular Carcinoma 307 samples same disease briefing
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Search all human RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.