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Sox11 overexpression restores embryonic pro-growth transcription in mature corticospinal tract neurons [snRNA-Seq]

GSE313126 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/12/10 Platform GPL34290
Summary
Neurons in the central nervous system (CNS) display a high capacity for axon growth during early development but mostly lose this ability during a key stage of differentiation marked by synaptic maturation, circuit integration, and a profound shift in gene transcription. Once mature, neurons largely fail to reverse this transcriptional switch after axon injury, fundamentally constraining their intrinsic capacity for repair. Here, we show with single-nucleus RNA sequencing that forced expression of the transcription factor Sox11 in adult corticospinal tract neurons produces large-scale and stable changes in gene expression that are highly enriched for growth-relevant processes, and which strongly resemble those of pre-synaptic embryonic stages. Moreover, Sox11 is equally effective when delivered to chronically injured CST neurons. These data reveal Sox11’s ability to reverse a critical step of neuronal maturation even in otherwise unperturbed neurons, clarifying the transcriptional underpinnings and highlighting Sox11’s potential as a pro-regenerative stimulus.
Published in
Sox11 overexpression restores embryonic pro-growth transcription in mature corticospinal tract neurons
Batsel E, Wang Z, Otten E et al. · Scientific reports 2025 · PMID 41436554 · doi:10.1038/s41598-025-33479-5
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Also filed as BioProject PRJNA1378573 and SRA study SRP653946. Searching any of these in the dataset finder brings you back here.

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