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Development of a human induced pluripotent stem cell-derived ovarian support cell clinical grade product for in vitro fertilization

GSE313528 Homo sapiens (human) RNA-seq (gene expression) 13 samples Submitted 2026/02/11 Platform GPL34284
At a glance

RNA-seq (gene expression) · human · 13 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
13 samples: 6 × RUO-OSC-M lot vs 3 × RUO-OSC-V lot vs 2 × RUO-OSC-L lot vs 2 × CG-OSC-L lotGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Development of human induced pluripotent stem cell-derived ovarian support cells as a clinical-grade product for in vitro fertilizationCell stem cell · 2026
Summary (written by the submitters)
Human induced pluripotent stem cells (hiPSCs) show promise in the development of novel strategies to alleviate reproductive diseases and improve reproductive outcomes. Here, we detail the clinical development and application of an ovarian support cell (OSC) product, Fertilo, to improve the in vitro maturation (IVM) of human oocytes. First, we demonstrate that transcription factor-mediated hiPSC differentiation produces OSCs that improve the oocyte MII maturation rate.
Read the full summary ↓
To support clinical application, we describe raw material upgrades and the generation of clinically-suitable hiPSC Seed and Master Cell Banks, with transcriptomic analysis of resultant OSCs showing consistent and reproducible outcomes. Next, we detail analytical release testing and development of a murine oocyte maturation assay to establish product potency. Finally, application of Fertilo in a longitudinal cohort analysis shows improvement in key outcomes compared to traditional IVM. Our findings demonstrate the first-time clinical development and application of an hiPSC-derived product to promote successful reproductive outcomes.
Published in
Development of human induced pluripotent stem cell-derived ovarian support cells as a clinical-grade product for in vitro fertilization
Paulsen B, Barrachina F, Piechota S et al. · Cell stem cell 2026 · PMID 41534521 · doi:10.1016/j.stem.2025.12.020
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE313528_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1380109 and SRA study SRP654735. Searching any of these in the dataset finder brings you back here.

Study design
6 × RUO-OSC-M lot vs 3 × RUO-OSC-V lot vs 2 × RUO-OSC-L lot vs 2 × CG-OSC-L lot

Supports a between-group comparison across 13 samples.

How we read this: 4 replicated groups read from 13 sample titles; they account for 13 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.