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Effect of chronic mechanical compression on immortalized human astrocytes

GSE313613 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/12/18 Platform GPL24676
Summary
Immortalized human astrocytes were subjected to a controlled in vitro model of chronic mechanical compression to investigate how solid stress—similar to that exerted by growing brain tumors—directly affects astrocyte transcriptional programs. RNA sequencing was performed after 24 hours of compression to measure sustained mechanotransductive responses. Compressed astrocytes showed robust induction of AP-1-associated immediate-early genes and activation of NF-κB–dependent inflammatory pathways. These data define the transcriptional signatures by which human astrocytes detect and respond to mechanical stress, providing a resource for understanding mechanobiology and neuroinflammatory signaling in diseases featuring mass effect, including glioblastoma and traumatic brain injury.
Published in
Mechanical compression induces neuronal apoptosis, reduces synaptic activity, and promotes glial neuroinflammation in mice and humans
Zarodniuk M, Wenninger A, Najera J et al. · Proceedings of the National Academy of Sciences of the United States of America 2026 · PMID 41481451 · doi:10.1073/pnas.2513172122
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Also filed as BioProject PRJNA1380395 and SRA study SRP654826. Searching any of these in the dataset finder brings you back here.

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