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Instant fluorescence lifetime imaging microscopy reveals mechano-metabolic reprogramming of stromal cells in breast cancer peritumor microenvironment

GSE313676 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/04/20 Platform GPL24247
Summary
The breast peritumor microenvironment (pTME) is increasingly recognized as a mediator of breast cancer progression and treatment resistance. However, how tumor compressive forces (i.e., solid stresses) influence it remains unclear. Using instant fluorescence lifetime imaging microscopy (FLIM), we show that in vitro compression metabolically reprograms stromal cells found in the breast pTME. Namely, compression shifts fibroblasts and differentiated adipocytes toward a more glycolytic state, but promotes increased oxidative phosphorylation in undifferentiated adipocytes. Through RNA-sequencing, we confirmed that compression downregulates oxidative phosphorylation and upregulates glycolysis in fibroblasts. By elucidating tumor-host mechano-metabolic interactions, these results will inform the development of innovative treatment strategies to improve survival.
Published in
Instant fluorescence lifetime imaging microscopy reveals mechano-metabolic reprogramming of stromal cells in breast peritumor microenvironment
Najera J, Chen H, Batista B et al. · iScience 2026 · PMID 42181268 · doi:10.1016/j.isci.2026.115931
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Also filed as BioProject PRJNA1380020. Searching any of these in the dataset finder brings you back here.

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