GEO series

GFI1 regulates NK cell maturation and function [scMultiome-seq].

GSE313719 Mus musculus Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2026/03/13 Platform GPL30172

Summary

Natural killer cells defend against malignancies and viral infections through a tightly controlled program of differentiation and maturation. However, the transcriptional mechanisms guiding this process remains incompletely defined. Using paired single cell multiomic profiling, we show that the transcriptional repressor GFI1 epigenetically regulates key molecular programs, including EOMES and T-BET balance, to promote NK cell proliferation and transition from immature to terminally differentiated NK stages. GFI1 was shown to repress FOXO1 chromatin accessibility in mature NK cells, which normally represses NK cell proliferation and maturation. Co-deletion of both GFI1 and FOXO1 largely rescued NK cell differentiation identifying a critical GFI1-FOXO1 axis essential for protection against tumour metastasis. These findings position GFI1 as an important transcriptional node integrating NK cell differentiation, activation, and effector programming.

Published in

The GFI1-FOXO1 axis regulates NK cell maturation and function

Huang Q, Chaudhry MZ, Dight J et al. · Nature communications 2026 · PMID 42020400 · doi:10.1038/s41467-026-72022-6

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Direct links to NCBI, no account and no request form: the whole study as GSE313719_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1381477 and SRA study SRP655195. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42020400) ↗

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