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CIPHER-seq enables low-stress intracellular multimodal profiling of immune activation

GSE314400 Homo sapiens Expression profiling by high throughput sequencing; Other 4 samples Submitted 2026/04/08 Platform GPL34281
Summary
This study introduces CIPHER-seq, an optimized intracellular CITE-seq workflow that minimizes fixation-induced cellular stress while enabling simultaneous measurement of intracellular proteins and transcriptomes in the same cell. Peripheral blood mononuclear cells (PBMCs) were processed using either CIPHER-seq or a commercial intracellular protocol (Proteintech) under unstimulated or PMA/ionomycin-stimulated conditions. Single-cell RNA and antibody-derived tag (ADT) libraries were generated using 10x Genomics Flex chemistry. The dataset enables benchmarking of intracellular chemistries, assessment of RNA–protein concordance, and characterization of cytokine-driven immune activation at single-cell resolution.
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Direct links to NCBI, no account and no request form: the whole study as GSE314400_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1390830 and SRA study SRP656538. Searching any of these in the dataset finder brings you back here.

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