← BioTransfer GEO Dataset Finder
GEO series

Targeting Modulated Vascular Smooth Muscle Cells in Atherosclerosis via FAP-Directed Immunotherapy [Mouse_Aorta_BiTE]

GSE314598 Mus musculus Other; Expression profiling by high throughput sequencing 6 samples Submitted 2026/02/02 Platform GPL24247
Summary
Vascular smooth muscle cell (VSMCs) cell diversification drives atherosclerotic coronary artery disease (CAD). Mechanisms governing these cell state transitions remain unclear. We applied multi-omic single-cell profiling, epitope mapping, and spatial transcriptomics across 27 human coronary arteries, identifying fibroblast activation protein (FAP) as a marker of modulated VSMCs. Lineage tracing in mice indicated that FAP⁺ cells originate from Myh11⁺ VSMCs, and FAP PET imaging in CAD patients showed plaque uptake. FAP⁺ cells states resided in the macrophage-rich neo-intima. Therapeutically, we developed an anti-FAP bispecific T-cell engager, which reduced plaque burden and remodeled the stromal–immune microenvironment through T-cell clonal expansion. Our study delivers a single-cell and spatial atlas of human CAD, establishes FAP as a marker of modulated VSMCs, and highlights immunotherapy for lipid-independent targets.
Published in
Targeting modulated vascular smooth muscle cells in atherosclerosis via FAP-directed immunotherapy
Amrute JM, Jung IH, Yamawaki T et al. · Science (New York, N.Y.) 2026 · PMID 41610194 · doi:10.1126/science.adx1736
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE314598_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1392196 and SRA study SRP657095. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all mouse datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.