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Oxygenated perfusion enhances hepatocyte function in human iPSC-liver tissue

GSE314639 Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2026/06/23 Platform GPL17303
Summary
This study aimed to create functional and scalable tissue from hiPSC-derived liver organoids (hiPSC-LOs) by establishing an oxygenation system utilizing a decellularized liver (DL), which retains the parenchymal and vascular extracellular matrix to support cell adhesion and medium perfusion. An oxygenation system for hiPSC-LO-engrafted DLs (hiPSC-LT) was established using perfusion of oxygen-enriched medium containing artificial red blood cells. Perfusion with oxygenated medium containing artificial red blood cells suppressed cell death and promoted hepatic function of hiPSC-LTs by mimicking the physiological oxygen concentration found in the fetal liver. The oxygenation system using DLs and artificial red blood cells effectively supported the generation of transplantable, functional hiPSC-LT.
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Direct links to NCBI, no account and no request form: the whole study as GSE314639_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1392355 and SRA study SRP657232. Searching any of these in the dataset finder brings you back here.

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