RNA-seq data of PEPD over-expressed vs NC GBM cells

Peptidase D (PEPD) is a cytosolic metallopeptidase previously reported to interact with nuclear p53, suppressing its phosphorylation and transcriptional activity to facilitate tumor progression in several cancer types, including bladder and colorectal cancer. However, the role of PEPD in glioma has not been described. Our preliminary proteomic analysis revealed that PEPD is markedly upregulated in glioma, with increased expression observed in both tumor tissues and tumor-supplying arterial blood, suggesting a potential involvement in glioma pathogenesis. To characterize the transcriptomic impact of PEPD in glioma, we conducted strand-specific RNA sequencing on two human glioblastoma cell lines following PEPD overexpression, comparing PEPD-overexpressing cells to their respective negative controls in both T98G and U251 models. Differential expression analysis was performed to identify PEPD-associated transcriptional alterations and to explore downstream signaling pathways and regulatory networks. These data provide a foundational resource for understanding PEPD-mediated molecular mechanisms and support further studies into its potential function as a previously unreported regulator in glioblastoma.