Effect of FADS2 overexpression on gene expression of adipose stem cells

In the current investigation, an RNA-seq analysis was conducted to examine the differentially expressed genes in aged ASCs (O-ASCs) following the overexpression of FADS2. The analysis revealed that 84 genes were significantly upregulated, while 516 genes were downregulated in oe-FADS2 O-ASCs compared to the oe-NC control group. GSEA indicated significant enrichment in pathways such as unsaturated fatty acid biosynthesis, the p53 signaling pathway, and the MAPK signaling pathway. GO analysis demonstrated that the differentially expressed genes were primarily localized to the nucleus and extracellular regions, with molecular functions encompassing metal ion binding and DNA binding activities, among others. Additionally, these genes were implicated in biological processes including angiogenesis, inflammatory response, and regulation of transcription mediated by RNA polymerase II. KEGG pathway analysis further highlighted enrichment in pathways associated with viral infection, cytokine-cytokine receptor interactions, the NOD-like receptor signaling pathway, and the IL-17 signaling pathway.