RXR binding pattern in human PMA-differentiated THP-1 cells upon ligand stimulation [RNA-seq]

Retinoid X receptors (RXRs) act as obligate dimerisation partners for multiple nuclear receptors (NRs), each with distinct regulatory functions. In this study, human PMA-differentiated THP-1 (PMA-THP-1) cells were treated with single or combined ligands for RXR or six partners for 2 hours, and the genomic binding landscape of RXR was investigated using ChIP-seq. RXR genomic binding regions were also mapped following 1 hour of treatment with vehicle or 1,25-vitD using ChIP-seq. In addition, histone H3 lysine 27 acetylation (H3K27ac) was mapped following 6 hours of treatment with vehicle or 1,25-vitD using ChIP-seq. In parallel, RNA-seq was performed on PMA-THP-1 cells following 6 hours of treatment with vehicle, 1,25-vitD or combined ligands to identify and compare the regulated gene sets.