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Dissecting polycomb complexes for enhanced phenotypic selectivity [RNA-Seq]

GSE316416 Homo sapiens (human) RNA-seq (gene expression) 9 samples Submitted 2026/05/14 Platform GPL30173
At a glance

RNA-seq (gene expression) · human · 9 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
9 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Dissecting polycomb complexes for enhanced fetal hemoglobin productionbioRxiv : the preprint server for biology · 2026
Summary (written by the submitters)
Polycomb repressive complexes PRC1 and PRC2 regulate diverse developmental processes, including the fetal-to-adult switch in hemoglobin production, a process whose reversal is a goal for the treatment of sickle cell disease and b-thalassemia. PRC inhibitors show promise for various disorders, but use is limited because of pleiotropic PRC activities.
Read the full summary ↓
We explored whether fetal hemoglobin (HbF) can be reactivated in adult erythroid cells by selective perturbations of PRC1 or PRC2 components without complete loss of PRC function. A high-density CRISPR-Cas9 mutagenesis screen identified a region in the EZH2 subunit where Cas9 induced exon 14 skipping (EZH2D14). EZH2D14, which lacks a portion of the CXC domain, relieves HbF repression while largely maintaining cellular fitness. EZH2D14 retains H3K27 methylation and repression of a PRC target gene subset. Experiments in mice bearing human b-globin genes confirm conserved EZH2 control of HbF expression. These findings demonstrate that partial disruption of PRC can yield selective phenotypes, highlighting the therapeutic potential of targeting non-enzymatic domains within chromatin-modifying complexes.
Published in
Dissecting polycomb complexes for enhanced fetal hemoglobin production
Kaminski PJ, Min K, Traxler EA et al. · bioRxiv : the preprint server for biology 2026 · PMID 42039476 · doi:10.64898/2026.04.16.718974
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE316416_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1402791 and SRA study SRP662109. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 9 more — browse all 9 samples with per-sample file links →

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