Transcriptomic profiling of epigenetic regulators and metabolic reprogramming in human cholangiocarcinoma [liver]

To study the expression of epigenetic and metabolic genes in cholangiocarcinoma (CCA) tumor progression, CCA was induced in C57BL/6J mice by hydrodynamic tail vein injection (HTVI) of plasmids coding for Notch Intracellular Domain 1 (NICD1, 10 µg per mouse) in combination with myr-AKT (10 µg/mouse) and the sleeping beauty transposase (NICD1AKT). C57BL/6J mice without HTVI injection were used as healthy controls (C). Mice were sacrificed at 2 and 3 weeks to evaluate the expression landscape of epigenetic and metabolic genes in tumor progression. We then performed gene expression profiling using RNA-seq data from 3 mice per group.