← BioTransfer GEO Dataset Finder
GEO series

Human liver-derived organoids recapitulate Oropouche virus infection and manifestation, enabling antiviral drug discovery

GSE316780 Homo sapiens (human) RNA-seq (gene expression) 32 samples Submitted 2026/01/21 Platform GPL20301
At a glance

RNA-seq (gene expression) · human · 32 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
32 samples: 8 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Human liver-derived organoids recapitulate Oropouche virus infection and manifestation, enabling antiviral drug discoveryCell reports. Medicine · 2026
Summary (written by the submitters)
Oropouche virus (OROV) is a neglected, re-emerging arbovirus that typically causes self-limiting febrile illness but can also result in severe complications. Currently, there are no approved vaccines or treatments for OROV infection. We integrated clinical data from OROV-infected patients with human liver-derived organoid models to investigate the virus's impact on the liver and evaluate repurposed antivirals.
Read the full summary ↓
Patient blood tests show elevated liver enzymes, suggesting OROV-associated hepatic dysfunction. OROV isolates productively infect liver organoids and induce severe cellular damage. Transcriptomic profiling reveals strong virus-host interactions, including activation of interferon-stimulated genes and cell death pathways. Pharmacological inhibition of the interferon pathway enhances OROV replication, whereas treatment with therapeutic interferon-α suppresses the infection. Molnupiravir, a clinically approved antiviral targeting viral RNA-dependent RNA polymerase, markedly inhibits OROV replication and mitigates virus-induced cytopathology. Combining molnupiravir with interferon-α resulted in synergistic antiviral activity, indicating the complementarity of virus-targeted and host-directed strategies.
Published in
Human liver-derived organoids recapitulate Oropouche virus infection and manifestation, enabling antiviral drug discovery
Li J, Wang X, Ding Y et al. · Cell reports. Medicine 2026 · PMID 41806841 · doi:10.1016/j.xcrm.2026.102646
This dataset
Open GSE316780 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE316780_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1404189 and SRA study SRP663624. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41806841) ↗
Study design
8 conditions, mostly with about 4 replicates each
Uninfected liver organoids, 96 h, ×4 OROV-1967 infected liver organoids, 1 h post-… ×4 OROV-1967 infected liver organoids, 48 h post… ×4 OROV-1967 infected liver organoids, 96 h post… ×4 OROV-2024 infected liver organoids, 1 h post-… ×4 OROV-2024 infected liver organoids, 48 h post… ×4 OROV-2024 infected liver organoids, 96 h post… ×4 OROV-2024 infected liver organoids, 1 μM NHC … ×4

Supports a between-group comparison across 32 samples.

How we read this: 8 replicated groups read from 32 sample titles; they account for 32 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.