RNA-seq analysis of PRCC-TFE3 dependent transcriptional programs and their modulation by MSC2530818 in HK-2 cells.

PRCC-TFE3 is an oncogenic chimeric transcription factor identified in TFE3-rearranged renal cell carcinoma. To characterize PRCC-TFE3–dependent transcriptional programs and their regulation by the Mediator kinase module, we performed RNA sequencing in a doxycycline-inducible PRCC-TFE3 expression system. HK-2 cells expressing HA-tagged PRCC-TFE3 under doxycycline control were cultured under four conditions defined by the presence or absence of doxycycline and the CDK8/19 inhibitor MSC2530818. RNA-seq was conducted to assess global gene expression changes associated with PRCC-TFE3 induction and CDK8/19 inhibition. These data provide a resource for analyzing PRCC-TFE3–driven transcriptional reprogramming and its modulation by the Cyclin C–CDK8/19 Mediator kinase module.