GEO series

The Potential Core Role of the FN1-Hippo Signaling Axis in the Pathological Mechanism of Autism spectrum disorder

GSE317721 Homo sapiens (human) RNA-seq (gene expression) 26 samples Submitted 2026/02/01 Platform GPL24676

At a glance

RNA-seq (gene expression) · human · 26 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 26 samples: 13 × Human, ASD group, vs 13 × Human, TD group,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Autism Spectrum Disorder (ASD) lacks reliable biomarkers and targeted therapies. This study integrated serum transcriptomics and proteomics from 13 ASD and 13 typically developing (TD) children to elucidate core molecular mechanisms. Differential expression analysis identified 6,563 genes (94.2% upregulated) and 157 proteins (75.2% downregulated), revealing widespread post-transcriptional dysregulation in ASD.

Read the full summary ↓

Functional enrichment demonstrated abnormalities in energy metabolism, ciliary function, and chemical perception pathways. O2PLS multi-omics integration nominated FN1 as a pivotal cross-omics target, exhibiting transcriptional elevation but proteomic reduction, indicating post-transcriptional perturbation. The Hippo signaling pathway emerged as a key regulatory hub connecting omics layers, with its core components (YAP1, MST1) significantly downregulated. Validation in a valproic acid-induced SH-SY5Y neuronal model confirmed that Hippo pathway inhibition drove FN1 transcriptional upregulation and selectively induced interleukin-6 (IL-6) expression, establishing a novel Hippo-FN1-IL-6 axis. This axis mechanistically links developmental signaling defects and neuroinflammation in ASD pathogenesis. Collectively, we define post-transcriptional dysregulation as a molecular hallmark of ASD and identify the Hippo-FN1-IL-6 cascade as a central pathogenic mechanism. FN1 represents a potential diagnostic biomarker and therapeutic target, providing novel insights for ASD intervention strategies.

This dataset

Open GSE317721 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE317721_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1415094 and SRA study SRP667693. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

13 × Human, ASD group, vs 13 × Human, TD group,

Supports a between-group comparison across 26 samples.

How we read this: 2 replicated groups read from 26 sample titles; they account for 26 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9477130 Human, ASD group,1 Human, ASD group,
GSM9477131 Human, ASD group,2 Human, ASD group,
GSM9477132 Human, ASD group,3 Human, ASD group,
GSM9477133 Human, ASD group,4 Human, ASD group,
GSM9477134 Human, ASD group,5 Human, ASD group,
GSM9477135 Human, ASD group,6 Human, ASD group,
GSM9477136 Human, ASD group,7 Human, ASD group,
GSM9477137 Human, ASD group,8 Human, ASD group,
GSM9477138 Human, ASD group,9 Human, ASD group,
GSM9477139 Human, ASD group,10 Human, ASD group,
GSM9477140 Human, ASD group,11 Human, ASD group,
GSM9477141 Human, ASD group,12 Human, ASD group,
GSM9477142 Human, ASD group,13 Human, ASD group,
GSM9477143 Human, TD group,1 Human, TD group,
GSM9477144 Human, TD group,2 Human, TD group,
GSM9477145 Human, TD group,3 Human, TD group,
GSM9477146 Human, TD group,4 Human, TD group,
GSM9477147 Human, TD group,5 Human, TD group,
GSM9477148 Human, TD group,6 Human, TD group,
GSM9477149 Human, TD group,7 Human, TD group,
GSM9477150 Human, TD group,8 Human, TD group,
GSM9477151 Human, TD group,9 Human, TD group,
GSM9477152 Human, TD group,10 Human, TD group,
GSM9477153 Human, TD group,11 Human, TD group,
GSM9477154 Human, TD group,12 Human, TD group,
GSM9477155 Human, TD group,13 Human, TD group,

Datasets on a similar topic

GSE307141 Investigating the Therapeutic Potential of the Antioxidant Epigallocatechin Gallate in Fetal Alcohol Spectrum Disorders: Cognition and RNASeq 16 samples shares potential, spectrum, disorder
GSE269105 Investigation of Chimeric Transcripts Derived from LINE-1 and Alu Retrotransposons in Cerebellar Tissues of Individuals with Autism Spectrum Disorder (ASD) 13 samples shares spectrum, disorder, autism
GSE292760 High-Throughput Drug Discovery for a Rare Neurological Disorder: Uncovering a Novel Therapeutic Opportunity for the 19q12 Autism Spectrum Disorder [post treatment entrectinib patient data] 10 samples shares spectrum, disorder, autism
GSE271853 Developmental convergence and divergence in human stem cell models of autism spectrum disorder 476 samples shares spectrum, disorder, autism
GSE298243 Single-Cell Profiling of DNA Methylation in Autism Spectrum Disorder Prefrontal Cortex Reveals Distinct Regulatory and Aging Signatures 49 samples shares spectrum, disorder, autism
GSE291257 Gene Expression Profiling Identifies Potential Biomarkers for Vaso-Occlusive Episodes in Sickle Cell Disease 414 samples shares potential
GSE308462 ALS molecular subtypes are a combination of cellular, genetic, and pathological features learned by deep multiomics classifiers (post-mortem cortex) 260 samples shares pathological
GSE135251 TRANSCRIPTOMIC PROFILING ACROSS THE SPECTRUM OF NON-ALCOHOLIC FATTY LIVER DISEASE 216 samples shares spectrum

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.