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Human epicardial organoids from pluripotent stem cells resemble fetal stage with potential cardiomyocyte-transdifferentiation

GSE317739 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2026/02/03 Platform GPL24676
Summary
Epicardium, the most outer mesothelium, exerts crucial functions in fetal heart development and adult heart regeneration. Here we use a three-step manipulation of WNT signalling entwined with BMP and RA signalling for generating a self-organized epicardial organoid that highly express with epicardium makers WT1 and TCF21 from human embryonic stem cells. After 8-days treatment of TGF-beta following by bFGF, cells enter into epithelium-mesenchymal transition and give rise to smooth muscle cells. Epicardium could also integrate and invade into mouse heart with SNAI1 expression, and give birth to numerous cardiomyocyte-like cells. Single-cell RNA seq unveils the heterogeneity and multipotency exhibited by epicardium-derived-cells and fetal-like epicardium. Meanwhile, extracellular matrix and growth factors secreted by epicardial organoid mimics the ecology of subepicardial space between the epicardium and cardiomyocytes. As such, this epicardial organoid offers a unique ground for investigating and exploring the potential of epicardium in heart development and regeneration
Published in
Human epicardial organoids from pluripotent stem cells resemble fetal stage with potential cardiomyocyte- transdifferentiation
Wang F, Zou X, Zheng H et al. · Cell & bioscience 2025 · PMID 39825425 · doi:10.1186/s13578-024-01339-w
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Also filed as BioProject PRJNA1415139 and SRA study SRP667769. Searching any of these in the dataset finder brings you back here.

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