E1A transactivation through superinfection turns iPSC-derived macrophages from a quiescent to a productive adenovirus cell state

Macrophages are a first line defense against pathogens. Human adenovirus C5 (AdV-C5) infection is inefficient in human iPSC-derived macrophages, partially due to poor activity of the enhancer/promoter (e/p) of the virus immediate early gene E1A. The E1A proteins are needed to activate other viral transcription units and to rewire the host cell for efficient virus replication. The E1A restriction can be overcome by coinfection of the wt virus together with a non-replicating AdV in which the E1A e/p has been replaced by the cytomegalovirus immediate early e/p (AdV-C5-CMV-E1A virus). We report here bulk RNA-seq results from noninfected iPSC-derived macrophages (NI samples), single wild type AdV-C5 (wt samples) and wt and AdV-C5-CMV-E1A coinfections (wtCMV samples) at 12 h and 27 h (marked as 24 h in the files) post infection. All samples had three technical replicates.