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MIB1-mediated H2AK119 mono-ubiquitination chromatin landscapes independent of canonical PRC1

GSE317825 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2026/02/09 Platform GPL24676
Summary
Polycomb repressive complex 1 (PRC1) is a central regulator of transcriptional repression through RING1B-mediated mono-ubiquitination of histone H2A at lysine 119 (H2AK119ub). Recent studies have suggested that the E3 ubiquitin ligase MIB1, previously characterized in Notch signaling, may also contribute to H2AK119ub deposition through a PRC1-independent mechanism. To investigate the chromatin-associated roles of MIB1, we generated ChIP-seq datasets for MIB1, RING1B, and H2AK119ub in the human colorectal cancer cell line HCT116. These datasets enable comparative analysis of genome-wide binding patterns, identification of shared and distinct target regions, and assessment of PRC1-dependent and PRC1-independent modes of H2AK119ub regulation.
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Direct links to NCBI, no account and no request form: the whole study as GSE317825_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1415369 and SRA study SRP668317. Searching any of these in the dataset finder brings you back here.

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