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Genome-wide EGR1 chromatin occupancy in wild-type C57BL/6J bone-marrow–derived macrophages measured by CUT-taq

GSE318298 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2026/02/05 Platform GPL24247
Summary
This study generated CUT-taq (tagmentation-based in situ ChIP; CUT&Tag-like) sequencing data to profile genome-wide EGR1 chromatin binding in mouse bone-marrow–derived macrophages (BMDMs). BMDMs were differentiated from wild-type C57BL/6J bone marrow in GM-CSF for 7 days, harvested at 4.0 × 10^5 cells per sample, and processed using the Hyperactive™ In-Situ ChIP Library Prep Kit for Illumina (Vazyme TD901–TD902). Cells were immobilized on concanavalin A beads, permeabilized with digitonin, incubated with anti-EGR1 primary and secondary antibodies (Thermo), and tagmented with Hyperactive pA-Tn5 (Vazyme). Libraries were PCR-amplified with P5/P7 primers, quality-checked on an Agilent 2100 Bioanalyzer, and sequenced on an Illumina NovaSeq 6000 (paired-end 150 bp). Raw sequencing files (FASTQ) and processed data files are provided to enable reuse and reanalysis of EGR1 binding profiles in GM-CSF–differentiated macrophages.
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Direct links to NCBI, no account and no request form: the whole study as GSE318298_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1418551 and SRA study SRP674390. Searching any of these in the dataset finder brings you back here.

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