GEO series
Ex vivo long-term expansion of human hematopoietic stem and progenitor cells as a tool for modeling vector integration sites and clonality
GSE318863
Homo sapiens
Expression profiling by high throughput sequencing
10 samples
2026/02/11
GPL18573GPL34284GPL24676
Summary
The small molecules A83-01, pomalidomide, and UM171 (APU) were used for the ex vivo expansion, lentiviral transduction, and long-term cultivation of umbilical cord blood-derived HSPCs. We determined the influence of APU on the stemness of HSPCs and their differentiation capacity via single-cell RNA sequencing (scRNA seq). APU supported the expansion of CD34+CD38-CD45RA-CD90+EPCR+ HSPCs. scRNAseq confirmed the enrichment of HSC signature genes in 24-h, 7-day, and 14-day APU-expanded HSPCs compared to the clinically used medium SFT3 (SCF, FLT3-L, TPO, IL-3).
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Paper (PMID 41654907) ↗
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