Chromatin accessibility in human colonic fibroblasts

We investigated genome-wide chromatin accessibility changes associated with replicative senescence in human colonic fibroblasts (HCoFs). A replicative senescence model was established by serial passaging of HCoFs, and cells at different stages of aging were collected: young (passage 6), mid-old (passage 15), and old (passage 23). To characterize age-associated alterations in chromatin structure, Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) was performed. Comprehensive epigenomic profiling revealed progressive remodeling of chromatin accessibility during replicative aging. Comparative analysis across young, mid-old, and old cells enabled identification of senescence-associated regulatory regions and dynamic changes in accessible chromatin landscapes. These data provide a valuable resource for understanding the epigenetic mechanisms underlying replicative senescence in human colonic fibroblasts and offer insight into how age-dependent chromatin reorganization contributes to transcriptional regulation during cellular aging.