GEO series

Transcriptomic Insights into Aerobic Exercise-Mediated Attenuation of High-Fat Diet–Induced Muscle Wasting

GSE319603 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2026/02/20 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 18 samples: 6 × control vs 6 × high-fat diet vs 6 × high-fat diet with MICT interventionGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Transcriptomic insights into aerobic exercise-mediated attenuation of high-fat diet-induced muscle wastingFrontiers in physiology · 2026

Summary (written by the submitters)

Chronic consumption of high-fat diet (HFD) induces obesity and metabolic dysfunction and is accompanied by progressive skeletal muscle wasting. Although aerobic exercise is generally considered less effective for maintaining muscle mass, accumulating evidence suggests that it can attenuate HFD-induced muscle wasting. However, the molecular mechanisms underlying this protective effect remain poorly defined.

Read the full summary ↓

In this study, we investigated the effects of moderate-intensity continuous training (MICT) on HFD-induced muscle wasting and characterized the associated transcriptomic adaptations in the mouse gastrocnemius muscle. Twenty-one weeks of HFD feeding increased body weight and serum glucose levels and induced marked muscle wasting, as evidenced by reduced gastrocnemius muscle index, impaired normalized forelimb grip strength, decreased muscle fiber cross-sectional area, and excessive intramuscular lipid accumulation. These pathological alterations were significantly attenuated by an 8-week MICT intervention. RNA sequencing revealed that HFD predominantly induced a lipid-centered transcriptional program characterized by enhanced fatty acid uptake, trafficking, and β-oxidation. In contrast, MICT predominantly suppressed atrophy-associated genes (Foxo1, Fbxo32, and Trim63), while exerting minimal effects on myogenic genes (Pax7, Myod1, and Myog). Functional enrichment analyses further indicated that MICT modulated signaling pathways related to FoxO, PI3K–Akt, MAPK, and insulin signaling, together with biological processes associated with angiogenesis and calcium signaling. Collectively, these results suggest that MICT mitigates HFD-induced muscle wasting primarily by reprogramming the transcriptome from a lipotoxic, atrophic state toward a more insulin-sensitive, pro-angiogenic profile, with limited myogenic activation.

Published in

Transcriptomic insights into aerobic exercise-mediated attenuation of high-fat diet-induced muscle wasting

Hong W, Zheng J, Luan Y et al. · Frontiers in physiology 2026 · PMID 41835150 · doi:10.3389/fphys.2026.1796142

This dataset

Open GSE319603 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE319603_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1424711 and SRA study SRP677622. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41835150) ↗

Study design

6 × control vs 6 × high-fat diet vs 6 × high-fat diet with MICT intervention

Supports a between-group comparison across 18 samples.

How we read this: 3 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9521100 control1 control
GSM9521101 control2 control
GSM9521102 control3 control
GSM9521103 control4 control
GSM9521104 control5 control
GSM9521105 control6 control
GSM9521106 high-fat diet1 high-fat diet
GSM9521107 high-fat diet2 high-fat diet
GSM9521108 high-fat diet3 high-fat diet
GSM9521109 high-fat diet4 high-fat diet
GSM9521110 high-fat diet5 high-fat diet
GSM9521111 high-fat diet6 high-fat diet
GSM9521112 high-fat diet with MICT intervention1 high-fat diet with MICT…
GSM9521113 high-fat diet with MICT intervention2 high-fat diet with MICT…
GSM9521114 high-fat diet with MICT intervention3 high-fat diet with MICT…
GSM9521115 high-fat diet with MICT intervention4 high-fat diet with MICT…
GSM9521116 high-fat diet with MICT intervention5 high-fat diet with MICT…
GSM9521117 high-fat diet with MICT intervention6 high-fat diet with MICT…

Datasets on a similar topic

GSE237747 Transcriptomic profiling across 20 CC-RIX genetic backgrounds with and without high-fat high-sugar (HFHS) diet, and with and without metformin [skeletal muscle] 703 samples shares high-fat, muscle, diet
GSE267727 Transcriptome profiling of non-islet metabolic tissues in ~500 DO mouse liver, adipose, heart, and skeletal muscle tissue from mice fed high-fat, high-sugar diet [heart] 482 samples shares high-fat, muscle, diet
GSE266569 Transcriptome profiling of non-islet metabolic tissues in ~500 DO mouse liver, adipose, heart, and skeletal muscle tissue from mice fed high-fat, high-sugar diet. [Liver] 481 samples shares high-fat, muscle, diet
GSE266567 Transcriptome profiling of non-islet metabolic tissues in ~500 DO mouse liver, adipose, and skeletal muscle tissue from mice fed high-fat, high-sugar diet. [skeletal muscle] 474 samples shares high-fat, muscle, diet
GSE266549 Transcriptome profiling of non-islet metabolic tissues in ~500 DO mouse liver, adipose, and skeletal muscle tissue from mice fed high-fat, high-sugar diet. [Adipose] 468 samples shares high-fat, muscle, diet
GSE266921 RNA-seq expression analysis of liver, adipose, skeletal muscle, heart, and pancreatic islets from DO founder strain mice fed high-fat, high-sugar diet. [Islet] 95 samples shares high-fat, muscle, diet
GSE266918 RNA-seq expression analysis of liver, adipose, skeletal muscle, heart, and pancreatic islets from DO founder strain mice fed high-fat, high-sugar diet. [heart] 63 samples shares high-fat, muscle, diet
GSE266923 RNA-seq expression analysis of liver, adipose, skeletal muscle, heart, and pancreatic islets from DO founder strain mice fed high-fat, high-sugar diet. [liver] 61 samples shares high-fat, muscle, diet

Search all mouse RNA-seq datasets in GEO →

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