GEO series

Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay

GSE31982 Homo sapiens; Escherichia coli Expression profiling by high throughput sequencing 18 samples Submitted 2012/02/26 Platform GPL11154Platform GPL14548

Summary

We apply a massively parallel reporter assay (MPRA) that relies on mRNA and plasmid tag sequencing (Tag-Seq) to compare the regulatory activities of more than 27,000 distinct variants of two inducible enhancers in human cells: a synthetic cAMP-regulated enhancer and the virus-inducible interferon beta enhancer. The resulting data define accurate maps of functional transcription factor binding sites in both enhancers at single-nucleotide resolution and can be used the to train quantitative sequence-activity models (QSAMs).

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Direct links to NCBI, no account and no request form: the whole study as GSE31982_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 18 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA145181 and SRA study SRP008145. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 22371084) ↗

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