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Netrin-1 blockade alleviates resistance to first line chemotherapy in locally advanced pancreatic cancer

GSE319924 Homo sapiens (human) RNA-seq (gene expression) 36 samples Submitted 2026/02/18 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 36 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Topic
Pancreatic cancerThis series is part of our curated briefing on it.
Samples
36 samples: 35 distinct titles, 1 of them repeatedGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Netrin1 blockade alleviates resistance to chemotherapy in pancreatic cancerNature · 2026
Summary (written by the submitters)
Netrin-1, a developmental cue, is a master regulator of tumor Epithelial-to-mesenchymal transition (EMT). Pancreatic cancer is a challenging indication where netrin-1 and its receptor neogenin have been shown to promote tumor progression and metastasis in animal models. Here we report the results of a single arm phase 1b study assessing the combination of an anti-netrin-1 antibody (NP137) with standard chemotherapy (mFOLFIRINOX) in first-line patients with locally advanced pancreatic cancer.
Read the full summary ↓
Fourty-three patients were included in this trial and received mFOLFIRINOX plus NP137 once every two weeks for up to 12 cycles (6 months). NP137 was well tolerated. Median PFS was 10.85 months (95% CI, 10.03 – 15.61) and median OS was 16.43 months (95% CI, 12.75 – NR) with 21 patients still alive at time of the data cut-off. Laser capture microdissection followed by microbulk RNA sequencing was performed on 22 pre-therapeutic and 6 surgery samples and revealed that the main pathway downregulated with the combination mFOLFIRINOX+NP137 is EMT, strengthening the mechanism of action of NP137. Moreover, analysis of pre-therapeutic samples revealed extended survival outcomes of patients with tumor cells expressing high levels of the netrin-1 receptor Neogenin, an observation validated by Neogenin immunostaining. Our results support netrin-1 blockade alleviates resistance to standard of care chemotherapy by inducing tumor re-epithelialization, particularly in neogenin-high pancreatic cancer.
Published in
Netrin1 blockade alleviates resistance to chemotherapy in pancreatic cancer
Roth G, Artru P, Bouche O et al. · Nature 2026 · PMID 42020751 · doi:10.1038/s41586-026-10436-4
This dataset
Open GSE319924 in finder → Find more human RNA-seq datasets → Pancreatic cancer landscape →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE319924_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1425531 and SRA study SRP678070. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42020751) ↗
Study design
35 distinct titles, 1 of them repeated
S022_Tumor_Post ×2

How we read this: Read from 36 sample titles: 35 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
More Pancreatic cancer datasets

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