Identifying Senescence and Immune Biomarkers Predictive of Benefit to Combined CDK4/6 and PD-1 Blockade in Dedifferentiated Liposarcoma: Single-Cell RNA-seq of Serial Tumor Biopsies
Single-cell RNA-seq · human · 23 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
- Organism
- Homo sapiens (human)
- Topic
- Soft tissue sarcomaThis series is part of our curated briefing on it.
- Samples
- 23 samples: 23 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Tumor and Immune Dynamics Following Sequential CDK4/6 and PD-1 Inhibition: Results from a Phase 2 Study in Dedifferentiated LiposarcomaCancer research communications · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE320212_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1427154. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 23 sample titles: 23 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9537699 P01 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537700 P02 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537701 P02 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 02)
- GSM9537702 P03 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 01)
- GSM9537703 P03 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 02)
- GSM9537704 P03 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537705 P03 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 02)
- GSM9537706 P05 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537707 P06 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537708 P07 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 01)
- GSM9537709 P07 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537710 P08 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 01)
- GSM9537711 P08 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 02)
- GSM9537712 P11 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537713 P11 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 02)
- GSM9537714 P12 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 01)
- GSM9537715 P12 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 02)
- GSM9537716 P12 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537717 P12 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 02)
- GSM9537718 P13 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 01)
- GSM9537719 P13 tumor biopsy scRNA-seq, C1D14 - palbociclib alone (rep 02)
- GSM9537720 P13 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 01)
- GSM9537721 P13 tumor biopsy scRNA-seq, C3D1 - palbociclib alone + IO (rep 02)
- GSE228127 Multimodal single-cell profiling reveals the enhanced antitumor activity of FGFR4/CD276 BiCisCAR T cells in rhabdomyosarcoma 12 samples same disease briefing · shares single-cell
- GSE325716 The MEK/MYC/p21 Axis Drives Senescence Bypass and BCL-xL Dependence in Vincristine-Resistant Rhabdomyosarcoma 4 samples same disease briefing · shares senescence
- GSE312030 Combined inhibition of HRAS and MEK induces tumor regression and restores myogenic differentiation in HRAS-mutant rhabdomyosarcoma 159 samples same disease briefing
- GSE273829 GABA promotes resistance to immunotherapy of patients with TLS-positive tumors [RNA-seq] 92 samples same disease briefing
- GSE300974 CDK8 Inhibition Releases the Muscle Differentiation Blockade in Fusion-driven Alveolar Rhabdomyosarcoma [RNA-seq] 30 samples same disease briefing
- GSE314949 RNA Seq of rhabdomyosarcoma cells with sgRNA targeting oncofusion interacting proteins 30 samples same disease briefing
- GSE279954 mRNA/sRNA Co-Sequencing Reveals Multiple New Targeted Therapy Candidates in Rhabdomyosarcoma 26 samples same disease briefing
- GSE292872 FANCM is Required for the PAX3::FOXO1-Driven Oncogenic Program in Rhabdomyosarcoma [RNA-Seq] 21 samples same disease briefing
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.