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8-Me-PIQ Expands Cord Blood HSCs via Metabolic Suppression and Inhibition of Ribosome Biogenesis

GSE320441 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/11 Platform GPL24676
Summary
8-methyl-4-((4-isopropylphenyl)amino)-[1,2,4]triazolo[4,3-a]quinoxaline (designated 8-Me-PIQ) is a potent activator of HSC expansion. To gain mechanistic insight into how 8-Me-PIQ promotes ex vivo expansion while preserving HSC stemness, we performed bulk transcriptomic analysis on UCB CD34+ cells cultured for 7 days in the presence of either 8-Me-PIQ or DMSO vehicle control.The convergence of transcriptional changes upon these specific biological processes suggests a coordinated mechanism by which 8-Me-PIQ preserves HSC stemness: attenuation of mitochondrial oxidative metabolism and reduction of global protein synthesis demand, thereby maintaining a quiescence-like state conducive to long-term function.
Published in
8-Me-PIQ Expands Cord Blood CD34(+) Hematopoietic Stem and Progenitor Cells via Metabolic Suppression and Inhibition of Ribosome Biogenesis
Li Y, Li M, Guo B et al. · Stem cell reviews and reports 2026 · PMID 41991719 · doi:10.1007/s12015-026-11115-7
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Also filed as BioProject PRJNA1428210 and SRA study SRP679261. Searching any of these in the dataset finder brings you back here.

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