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Effect of SREBP1 cDNA overexpression on gene expression in LN229 cells

GSE320462 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/04/16 Platform GPL34284
Summary
Aberrant activation of the PI3K/AKT/mTOR signaling pathway is a common feature of cancer but, while mTOR kinase represents an attractive drug target, mTOR inhibitors have not seen broad success as single agents. To identify strategies to enhance the utility of mTOR inhibitors we performed forward genetic screens with new bi-steric mTORC1 inhibitors. These screens show that mTORC1 inhibitor-mediated cytostasis leaves cells exquisitely dependent on the lipid peroxide scavenging enzyme GPX4. Mechanistically, using unbiased gene activation screens and RNA-seq, we demonstrate that mTORC1-dependent control of ferroptosis occurs, in part, through regulation of SREBP1 and donwstream impact on SCARB1 expression.
Published in
mTORC1 activity suppresses ferroptosis through a SCARB1-dependent HDL-tocopherol uptake pathway
O'Loughlin TA, Stiles JS, Acharya P et al. · Molecular cell 2026 · PMID 41997112 · doi:10.1016/j.molcel.2026.03.019
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Also filed as BioProject PRJNA1428246 and SRA study SRP679304. Searching any of these in the dataset finder brings you back here.

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