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Investigation of epigenetic and transcriptomic features of human B cell development [Multiome]

GSE322590 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing 8 samples Submitted 2026/05/14 Platform GPL16791
Summary
This dataset was generated as part of a multi-omics study aimed at investigating human B cells development. The study included bulk and single cell epigenetic methodologies coupled with single cell transcriptomics and proteomics. Data included in this submission were generated using the 10x Chromium Single Cell Multiome ATAC + Gene Expression method to allow simultaneous investigation of epigenetic and transcriptomic features in sorted B cells from human blood. The study demonstrated the emergence of epigenetic differences in B cell subsets at early stages of development that drive divergent developmental paths. Epigenetic features established at early stages are retained in distinct mature cell subpopulations, where they are coupled with transcription of accessible genomic regions. Identified features are retained after cell migration in secondary lymphoid organs.
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Direct links to NCBI, no account and no request form: the whole study as GSE322590_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1430645 and SRA study SRP680272. Searching any of these in the dataset finder brings you back here.

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