Feeder-Free Generation of Lymphatic Endothelial Cells from Human Induced Pluripotent Stem Cells

The lymphatic system is vital for fluid balance, lipid transport, and immune function, but studying human lymphatic vasculature has been limited by poor access to primary LECs. Here, we present an efficient, scalable, feeder-free protocol to differentiate human iPSCs into iLECs that closely resemble primary fetal LECs transcriptionally and phenotypically, enabling disease-relevant studies and precise genetic perturbations supported by marker validation and bulk RNA-seq comparisons to human fetal endothelial datasets.