Characterize the effects of L. asaccharolyticus on autistic-like symptoms

Accumulating evidence has revealed a strong link between the gut microbial community and ASD symptoms mediated by the gut–brain axis; however, ASD-specific microbial markers and the underlying mechanisms for targeted interventions remain unclear. Based on our previous identification of Lawsonibacter asaccharolyticus loss as a candidate ASD marker, we performed bacterial intervention in the BTBR ASD mouse model (C57 mice as healthy controls). Bulk RNA-seq was conducted on brain cortex and colon sections. In vitro validation: SH-SY5Y neuronal cells were treated for 24 h with bacterial filtrate, its derivative metabolite oleoylethanolamide (OEA), or butyrate (BA); untreated cells served as controls. All samples were subsequently analyzed by bulk RNA-seq.