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RFX5 Mediates High-order Structures to Regulate Cancer Progression-Associated Genes [ATAC-seq]

GSE325108 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2026/05/05 Platform GPL34281
Summary
RFX5 is a DNA binding protein known to regulate MHC genes expressions with little studies on its role in maintaining 3D genome architecture. Recent studies have shown its potential in mediating long-range interactions in specific regions, though the underlying mechanism remains poorly understood. Here, using genes related to cancer progression as a paradigm, we studied the role of RFX5 and found that it specifically regulates these genes. It mediates interactions between gene promoters and their distal enhancers, which can be several hundreds of kilobases apart. We also found that RFX5 regulates higher-order structures of TADs and compartments, which become unstable upon loss of RFX5. Our findings elucidate the fundamental role of RFX5 in maintaining proper chromosome architecture and shed light on the relationship between chromatin loops and gene expression.
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Direct links to NCBI, no account and no request form: the whole study as GSE325108_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1438655 and SRA study SRP684483. Searching any of these in the dataset finder brings you back here.

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