GEO series

Restraint of Powassan virus replication by TRIM5a facilitates viral avoidance of antiviral immunity

GSE325334 Homo sapiens (human) RNA-seq (gene expression) 30 samples Submitted 2026/06/17 Platform GPL24676

At a glance

RNA-seq (gene expression) · human · 30 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 30 samples: 30 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

TRIpartite Motif (TRIM) protein 5 alpha (TRIM5a) is a well characterized cellular inhibitor of lentivirus replication that limits transmission of related viruses between primates. We previously reported that TRIM5a derived from humans and rhesus macaques inhibits replication of orthoflaviviruses belonging to the tick-borne encephalitis virus (TBEV) serocomplex, including TBEV, Kyasanur forest disease virus and Langat virus (LGTV), but interestingly not the tick-borne Powassan virus (POWV).

Read the full summary ↓

To further characterize the primate TRIM5a and orthoflavivirus interface, we screened TRIM5a variants from representative old- and new-world primates for restriction capacity. TRIM5a from old-world African green monkey, De Brazza’s monkey and chimpanzee demonstrated virus-specific restriction of tick-borne orthoflaviviruses. Efforts to determine why TRIM5a fails to inhibit POWV revealed that our lab stock had acquired a non-synonymous mutation in NS3 that, when introduced into a POWV molecular clone, facilitated virus replication in the presence of all inhibitory primate TRIM5a proteins. Infection of human dendritic cells with TRIM5a-resistant POWV resulted in high early replication and strong induction of interferon responses that limited replication compared with the wild-type virus. Thus, primate TRIM5a functions as a potent cellular barrier to infection with tick-borne orthoflaviviruses that restrains replication to a level that may help avoid early innate immune recognition.

This dataset

Open GSE325334 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE325334_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1439725 and SRA study SRP685031. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

30 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 30 sample titles: 30 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9600920 Donor1, MOCK Group, 24 Hour Timepoint
GSM9600922 Donor2, MOCK Group, 24 Hour Timepoint
GSM9600924 Donor3, MOCK Group, 24 Hour Timepoint
GSM9600926 Donor4, MOCK Group, 24 Hour Timepoint
GSM9600928 Donor5, MOCK Group, 24 Hour Timepoint
GSM9600931 Donor1, WT Group, 24 Hour Timepoint
GSM9600933 Donor2, WT Group, 24 Hour Timepoint
GSM9600935 Donor3, WT Group, 24 Hour Timepoint
GSM9600937 Donor4, WT Group, 24 Hour Timepoint
GSM9600939 Donor5, WT Group, 24 Hour Timepoint
GSM9600940 Donor1, T651M Group, 24 Hour Timepoint
GSM9600941 Donor2, T651M Group, 24 Hour Timepoint
GSM9600942 Donor3, T651M Group, 24 Hour Timepoint
GSM9600943 Donor4, T651M Group, 24 Hour Timepoint
GSM9600944 Donor5, T651M Group, 24 Hour Timepoint
GSM9600945 Donor1, MOCK Group, 48 Hour Timepoint
GSM9600946 Donor2, MOCK Group, 48 Hour Timepoint
GSM9600947 Donor3, MOCK Group, 48 Hour Timepoint
GSM9600948 Donor4, MOCK Group, 48 Hour Timepoint
GSM9600949 Donor5, MOCK Group, 48 Hour Timepoint
GSM9600950 Donor1, WT Group, 48 Hour Timepoint
GSM9600951 Donor2, WT Group, 48 Hour Timepoint
GSM9600952 Donor3, WT Group, 48 Hour Timepoint
GSM9600953 Donor4, WT Group, 48 Hour Timepoint
GSM9600954 Donor5, WT Group, 48 Hour Timepoint
GSM9600955 Donor1, T651M Group, 48 Hour Timepoint
GSM9600956 Donor2, T651M Group, 48 Hour Timepoint
GSM9600957 Donor3, T651M Group, 48 Hour Timepoint
GSM9600958 Donor4, T651M Group, 48 Hour Timepoint
GSM9600959 Donor5, T651M Group, 48 Hour Timepoint

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