← BioTransfer GEO Dataset Finder
GEO series

Multi-omic analysis reveals brain-blood concordance and persistent neuroimmune reprogramming with stress and toxicant exposure in a mouse model of Gulf War Illness [RNA-Seq]

GSE325692 Mus musculus (mouse) RNA-seq (gene expression) 30 samples Submitted 2026/03/27 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 30 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
30 samples: 8 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Multi-omic analysis reveals brain-blood concordance and persistent neuroimmune reprogramming with stress and toxicant exposure in a mouse model of Gulf War IllnessBrain, behavior, & immunity - health · 2026
Summary (written by the submitters)
Gulf War Illness (GWI) is a chronic multisymptom disorder associated with prior stress and toxicant exposures. Adult male C57BL/6J mice were exposed to corticosterone (CORT) and diisopropyl fluorophosphate (DFP), followed by repeated intermittent CORT and a later immune challenge with lipopolysaccharide (LPS) or saline. RNA-seq was performed in hippocampus and frontal cortex collected 6 h, 12 h, and 24 h after challenge to define transcriptional responses associated with persistent neuroimmune priming in this mouse model of GWI.
Published in
Multi-omic analysis reveals brain-blood concordance and persistent neuroimmune reprogramming with stress and toxicant exposure in a mouse model of Gulf War Illness
Sasaki A, Kelly KA, Michalovicz LT et al. · Brain, behavior, & immunity - health 2026 · PMID 41993916 · doi:10.1016/j.bbih.2026.101207
This dataset
Open GSE325692 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE325692_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1441474 and SRA study SRP685936. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41993916) ↗
Study design
8 conditions, mostly with about 4 replicates each
Hippocampus, CORT+DFP+LPS, 6 h, A ×4 Frontal cortex, CORT+DFP+LPS, 6 h, A ×4 Hippocampus, CORT+DFP+LPS, 12 h, A ×4 Frontal cortex, CORT+DFP+LPS, 12 h, A ×4 Hippocampus, CORT+DFP+LPS, 24 h, A ×4 Frontal cortex, CORT+DFP+LPS, 24 h, A ×4 Hippocampus, CORT+DFP+Saline, 6 h, A ×3 Frontal cortex, CORT+DFP+Saline, 6 h, A ×3

Supports a between-group comparison across 30 samples.

How we read this: 8 replicated groups read from 30 sample titles; they account for 30 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.