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Single-cell RNA sequencing reveals blood contact-dependent functional specialization of vascular-associated immune cells in a MCAO model

GSE326055 Mus musculus (mouse) Single-cell RNA-seq 4 samples Submitted 2026/10/01 Platform GPL21103
At a glance

Single-cell RNA-seq · mouse · 4 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism
Mus musculus (mouse)
Samples
4 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
We integrated intravascular CD45 staining with scRNA-seq to dissect spatial-functional heterogeneity of immune cells in a murine stroke model- middle cerebral artery occlusion (MCAO). Data identify blood contact status as a key driver of immune cell specialization, providing insights into mechanisms of the ischemic stroke.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE326055_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1443237. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 4 more — browse all 4 samples with per-sample file links →

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