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Investigation of epigenetic features of human B cells

GSE326191 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 29 samples Submitted 2026/05/14 Platform GPL16791
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 29 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
29 samples: 9 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
This dataset was generated as part of a multi-omics study aimed at investigating human B cells development. The study included bulk and single cell epigenetic methodologies coupled with single cell transcriptomics and proteomics. Data in this submission include sequenced libraries for bulk ATAC-seq and for H3K4me3 CUT&RUN. Samples include sorted transitional and mature B cell populations from human adult and cord blood.
Read the full summary ↓
The study demonstrated the emergence of epigenetic differences in B cell subsets at early stages of development that discriminate between divergent developmental paths. Epigenetic features established at early stages are retained in distinct mature cell subpopulations, where they are coupled with transcription of accessible genomic regions.
This dataset
Open GSE326191 in finder → Find more human ChIP / ATAC / CUT&Tag datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE326191_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1444096 and SRA study SRP687214. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
9 conditions, mostly in triplicate
Bulk ATAC-seq, B-cells, Adult blood, TS cells… ×3 Bulk ATAC-seq, B-cells, Adult blood, TS cells… ×3 Bulk ATAC-seq, B-cells, Adult blood, TS cells… ×3 Bulk ATAC-seq, B-cells, Adult blood, Mature c… ×3 Bulk ATAC-seq, B-cells, Adult blood, Mature c… ×3 Bulk ATAC-seq, B-cells, Cord blood, TS cells,… ×3 Bulk ATAC-seq, B-cells, Cord blood, TS cells,… ×3 Bulk CUTandRUN, B-cells, Adult blood, TS cell… ×2 +5 more

Supports a between-group comparison across 25 samples.

How we read this: 9 replicated groups read from 29 sample titles; they account for 25 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.