in vivo human embryonic spinal cord atlas validates stem cell-derived human dorsal interneurons and reveals ASD spinal signatures [day0NMP_bulk RNAseq]
Single-cell RNA-seq · human · 15 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Homo sapiens (human)
- Samples
- 15 samples: 5 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- In vivo human embryonic spinal cord atlas validates stem cell-derived human dorsal interneurons and reveals ASD spinal signaturesbioRxiv : the preprint server for biology · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE326651_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1446684 and SRA study SRP688439. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 15 samples.
How we read this: 5 replicated groups read from 15 sample titles; they account for 15 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9636525 2D NMP, rep 1 2D NMP,
- GSM9636526 2D NMP, rep 2 2D NMP,
- GSM9636527 2D NMP, rep 3 2D NMP,
- GSM9636528 4D NMP, rep 1 4D NMP,
- GSM9636529 4D NMP, rep 2 4D NMP,
- GSM9636530 4D NMP, rep 3 4D NMP,
- GSM9636531 10D NMP, rep 1 10D NMP,
- GSM9636532 10D NMP, rep 2 10D NMP,
- GSM9636533 10D NMP, rep 3 10D NMP,
- GSM9636534 10D GDF11 NMP, rep 1 10D GDF11 NMP,
- GSM9636535 10D GDF11 NMP, rep 2 10D GDF11 NMP,
- GSM9636536 10D GDF11 NMP, rep 3 10D GDF11 NMP,
- GSM9636537 hESC control, rep 1 hESC control,
- GSM9636538 hESC control, rep 2 hESC control,
- GSM9636539 hESC control, rep 3 hESC control,
- GSE326655 in vivo human embryonic spinal cord atlas validates stem cell-derived human dorsal interneurons and reveals ASD spinal signatures [day43NMP_scRNAseq] 12 samples shares asd, cell-derived, interneurons, signatures, embryonic, validates, spinal
- GSE326654 in vivo human embryonic spinal cord atlas validates stem cell-derived human dorsal interneurons and reveals ASD spinal signatures [day27NMP_scRNAseq] 8 samples shares asd, cell-derived, interneurons, signatures, embryonic, validates, spinal
- GSE244189 Transcriptomic analysis of ASD and IDD associated pathogenic MYT1L S707Q mutation in human cortical interneurons 24 samples shares asd, interneurons
- GSE226482 Single cell RNA sequencing of spinal motor neurons and interneurons differentiated from isogenic FUS and TARDBP human induced pluripotent stem cell lines harbouring ALS-causative mutations 1415 samples shares interneurons, spinal
- GSE305933 Temporal multi-omics gene expression data across human embryonic stem cell-derived polyhormonal cell differentiation [RNA-seq] 20 samples shares cell-derived, embryonic
- GSE249992 Bulk and single-cell transcriptomics identify gene signatures of stem cell-derived NK cell donors with superior anti-tumor activity 17 samples shares cell-derived, signatures
- GSE249991 Bulk and single-cell transcriptomics identify gene signatures of stem cell-derived NK cell donors with superior anti-tumor activity [Bulk RNA-seq] 16 samples shares cell-derived, signatures
- GSE275825 Transcriptome analysis of human embryonic stem cell-derived cartilage pellets at three stages of differentiation 14 samples shares cell-derived, embryonic
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.