GPR34 regulation of disease-associated microglial states in mice

We investigated the role of the microglia-enriched G protein–coupled receptor GPR34 in regulating cell states for microglia and other cell types under physiological and disease conditions using single-cell RNA sequencing (scRNA-seq) and single-nuclei RNA sequencing (snRNA-seq). Microglia isolated from Gpr34 knockout (KO) and wild-type mice, with or without the 5xFAD Alzheimer’s disease background, were profiled to assess transcriptional changes associated with GPR34 loss, as were other cell types in the hippocampus. Our findings indicate that GPR34 plays a critical role in maintaining microglial homeostasis and restraining disease-associated transcriptional responses in vivo.