← BioTransfer GEO Dataset Finder
GEO series

Mutual interaction between Schwann cells and CD4+T cells promotes the progression of perineural invasion in colorectal cancer [ATAC-seq]

GSE327650 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 7 samples Submitted 2026/06/30 Platform GPL9052
Summary
Background: Perineural invasion (PNI) is a common pathological feature associated with poor prognosis of colorectal cancer (CRC). However, the mechanisms underlying PNI formation and its relationship with CD4+T cells within the tumor immune microenvironment remain unclear. Aims: This study investigated the role of CD4+T cells in the development and progression of PNI in CRC by examining the interactions between Schwann cells (SCs), primary glial cells in peripheral nerves, and CD4+T cells. Methods: Single-cell RNA sequencing, spatial transcriptomics, and multiplex immunohistochemistry were used to profile the characteristics and spatial relationship between SCs and CD4+T cells in PNI CRC tissues. This study established an SCs-CD4⁺T-cells co-culture system and used flow cytometry, transcriptomic sequencing, proteomics, and ATAC-seq to investigate the mechanisms of their interaction. The key findings were validated in mouse models of sciatic nerve invasion and subcutaneous tumor formation. Finally, the origin of PNI was explored by co-culturing SCs with CRC cells.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE327650_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1451762 and SRA study SRP690854. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 7 more — browse all 7 samples with per-sample file links →

Similar datasets

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.