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The PGD2/DP2 axis promotes pro-inflammatory responses but attenuates an antigen-presenting role of human basophils

GSE328453 Homo sapiens (human) RNA-seq (gene expression) 24 samples Submitted 2026/04/29 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
24 samples: 24 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
The PGD(2)/DP2 axis promotes pro-inflammatory responses but attenuates an antigen-presenting role of human basophilsCommunications biology · 2026
Summary (written by the submitters)
Basophils are distinct blood leukocytes that express immunoglobulin (Ig) E receptor FcR1 and can be activated by IgE. Their roles in asthma, particularly IgE-independent effects, remain incompletely understood. Human basophils highly express the prostaglandin D2 (PGD2) receptor 2 (DP2; also known as chemoattractant receptor homologous molecule expressed on Th2 cells, CRTH2).
Read the full summary ↓
Here we explore the PGD2/DP2 axis as an alternative pathway for basophil activation. Basophils are enriched in patients with severe eosinophilic asthma, correlating with eosinophil counts but not IgE levels. PGD2/DP2 stimulation promots basophil recruitment, activation, and the production of histamine, leukotrienes, and type 2 cytokines. RNA sequencing and further in vitro experiments reveals that, although PGD2 and IgE share some signalling pathways and functions, they induce distinct gene transcriptional signatures. Interestingly, PGD2 downregulats major histocompatibility complex class I-related gene protein (MR1) and attenuates basophil antigen-presentation to mucosal associated invariant T (MAIT) cells, leading to reduced IFN- production. These findings highlight the PGD2/DP2/basophil axis as a contributor to asthma, particularly in IgE-low conditions, and suggest it as a potential therapeutic target for related diseases.
Published in
The PGD(2)/DP2 axis promotes pro-inflammatory responses but attenuates an antigen-presenting role of human basophils
Luo J, Chen W, Lett MJ et al. · Communications biology 2026 · PMID 42162122 · doi:10.1038/s42003-026-10286-w
This dataset
Open GSE328453 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE328453_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1455475 and SRA study SRP693113. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42162122) ↗
Study design
24 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 24 sample titles: 24 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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