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Comparative transcriptomic profiling of TRI-LC21 and BEAS-2B cells to investigate SMARCA4-deficient undifferentiated tumor

GSE328872 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/07/15 Platform GPL24676
Summary
This study aimed to characterize the transcriptomic features of TRI-LC21. Due to the limited availability of well-defined in vitro models for this tumor type, we sought to investigate gene expression changes associated with SMARCA4 loss and tumor dedifferentiation. RNA sequencing was performed to compare TRI-LC21 with the normal bronchial epithelial cell line BEAS-2B. Differential expression analysis revealed extensive transcriptional alterations, including activation of neuron-associated pathways and suppression of interferon and p53 signaling. In addition, TRI-LC21 exhibited decreased expression of epithelial markers and increased expression of stemness- and epithelial–mesenchymal transition-related genes, consistent with a poorly differentiated transcriptional state. These data provide insight into the molecular mechanisms underlying SMARCA4-deficient tumors and support the utility of TRI-LC21 as an in vitro model for further biological and therapeutic studies.
Published in
Establishment and characterization of TRI-LC21: a novel patient-derived cell line of SMARCA4-deficient undifferentiated thoracic tumor
Li S, Luo H, Wu D et al. · Human cell 2026 · PMID 42426436 · doi:10.1007/s13577-026-01418-9
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Also filed as BioProject PRJNA1456917 and SRA study SRP694171. Searching any of these in the dataset finder brings you back here.

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