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SMART-seq profiling of the ascending aorta and aortic arch from male and female Wnt1-Cre;Rosa26-mTmG mice

GSE329247 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/05/03 Platform GPL34290
Summary
Vascular smooth muscle cells (VSMCs) in the ascending aorta and aortic arch arise from two distinct embryonic origins: the second heart field (SHF) and the cardiac neural crest (CNC). Whether these populations exhibit sex-dependent differences remains unknown. Here, we used Wnt1-Cre;Rosa26-mTmG mice to genetically label CNC-derived VSMCs with GFP, while non-CNC-derived cells expressed tdTomato. The ascending aorta and aortic arch were dissected from male and female mice, and single-cell suspensions were generated by enzymatic digestion. Cells were then sorted by fluorescence-activated cell sorting (FACS) based on GFP or tdTomato expression. RNA-expression profiles for each group were obtained using the Smart-seq platform. Thi study revealed the differential expression of VSMCs from different embryonic origin in both sexes.
Published in
Single-cell lineage tracing unveils sex differences in VSMCs derived from the cardiac neural crest and second heart field
Wang Y, Luo C, Wang S et al. · Vascular pharmacology 2026 · PMID 42069076 · doi:10.1016/j.vph.2026.107611
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Also filed as BioProject PRJNA1458202 and SRA study SRP695304. Searching any of these in the dataset finder brings you back here.

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