Endothelial transcriptome remodeling by young and senescent MSCs depends on co-culture mode, ECM, and secreted factors

Increasing longevity and the growing elderly population necessitate a deeper understanding of cellular interactions within the aging microenvironment, particularly those involving mesenchymal stromal cells (MSCs) and endothelial cells (ECs), as their cross-talk is critical for tissue homeostasis and regeneration. However, how endothelial cells differentially respond to young versus senescent MSCs depending on the mode of interaction – whether through extracellular matrix (ECM) deposition, secreted factors, or direct cell-cell contact – remains poorly characterized. This study aims to provide a transcriptomic dataset of endothelial cells following co-culture with young (mitomycin‑untreated) and senescent (mitomycin‑treated) MSCs under three distinct conditions: on MSC‑derived ECM, in MSC‑conditioned medium, and in direct co‑culture. The RNA‑sequencing data will enable comparative analysis of endothelial transcriptional programs modulated by proliferating versus senescent MSCs across different compartments of the MSC-EC niche.