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Multi-omic profiling of human pluripotent cells derived by nuclear transfer and defined factors (H3K27ac ChIP-seq)

GSE330333 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2026/05/13 Platform GPL18573
Summary
A panel of nine human cell lines comprising in vitro fertilization-derived embryonic stem cells (hESCs), nuclear-transfer embryonic stem cells (NT-ESCs; isogenic with the donor fibroblasts), induced pluripotent stem cells (iPSCs; isogenic with the donor fibroblasts) and parental dermal fibroblasts (hDFs) was profiled to compare the transcriptional and epigenetic landscapes that arise from different reprogramming routes. The four sequencing assays (transcriptome RNA-seq, MeDIP-seq for DNA methylation, H3K27ac ChIP-seq for active enhancer annotation and small RNA-seq for microRNA expression) were generated for the same set of donors so as to enable an integrative four-layer comparison. NT-ESCs and iPSCs largely recapitulate the core pluripotency network of hESCs but retain residual donor-cell signatures, and the method-of-derivation effect is most pronounced at the DNA methylation and microRNA layers.
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Direct links to NCBI, no account and no request form: the whole study as GSE330333_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1463670 and SRA study SRP698639. Searching any of these in the dataset finder brings you back here.

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