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Transcriptional Response to WNT-inhibition-induced human cardiomyogenesis (single-cell RNA-seq)

GSE333970 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/06/08 Platform GPL18573
Summary
Since embryonic cardiac development in vivo and in vitro cardiomyocyte differentiation protocols both require WNT inhibition, our study focussed on how inhibition of WNT signalling guides the transcriptional landscape towards human cardiomyocyte differentiation. We uncovered transcriptional responses using single-cell RNA sequencing. with bioinformatics analysis defining cell identities, reconstructing differentiation trajectories, dissecting WNT inhibition-dependent gene expression and inferring Gene Regulatory Networks (GRN) driving cardiomyocyte specification. We found that WNT inhibition (WNTi) redirects early mesoderm precursors towards a cardiomyocyte fate while limiting alternative lineages.
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Direct links to NCBI, no account and no request form: the whole study as GSE333970_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1473305 and SRA study SRP705851. Searching any of these in the dataset finder brings you back here.

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