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Sympathetic Neurons Restrain Pancreatic Cancer Lung Metastatic seeding through NPY-NPY1R Signaling [barcode]

GSE334016 Mus musculus (mouse) Other 11 samples Submitted 2026/08/06 Platform GPL34290
At a glance

mouse · 11 samples

Organism
Mus musculus (mouse)
Samples
11 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI.
Summary (written by the submitters)
The sympathetic nervous system regulates physiological homeostasis, but is increasingly recognized as a context dependent modulator of carcinogenesis. Here, we investigate the function of sympathetic innervation on pancreatic ductal adenocarcinoma (PDAC) metastasis. Although sympathetic signaling has been shown to promote primary PDAC growth, its contribution to metastatic progression remains poorly defined.
Read the full summary ↓
Using a pool of barcoded Kras;Trp53-driven mouse PDAC cell lines that allows quantitative analysis of metastatic seeding and growth, we found that sympathetic innervation restrains metastatic seeding but promotes metastatic outgrowth. In vivo pharmacologic screening showed that inhibition of the Neuropeptide Y (NPY) receptor NPY1R phenocopied the effect of sympathetic ablation on metastatic seeding, indicating sympathetic release of NPY restrains metastatic seeding through NPY1R signaling, likely in lung stromal cells including alveolar fibroblasts. Single-cell transcriptional analyses revealed that NPY1R signaling affects alveolar fibroblasts and intermediate gCap endothelial cells, providing a mechanism for restraining vascular permeability and cancer cell seeding. Together, these findings uncover an unexpected metastasis-protective function of sympathetic innervation, distinct from its effect on metastatic growth, and identify a novel sympathetic-stromal signaling axis that regulates the vascular niche during PDAC lung metastasis.
This dataset
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Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗
3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE334016_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

Whole study · RAW.tar ↓

Also filed as BioProject PRJNA1473586. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
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